Scireq inExpose system for exposing subjects to different substances

inExpose: system for exposing subjects to different substances

inExpose: system for exposing subjects to different substances

inExpose (Scireq Inc) is an integrated and compact tool for exposing subjects to profiles of pharmacological compounds, cigarette smoke and other toxins:

The modular design allows adaptation to your needs. Each system has:

• a base unit, incorporating pump and electronic controller
• an exposure subsystem (see below)
• optional components, such as nebulization or smoke generating device

features

• precise and efficient control of the exposure process, nebulization rates
• a wide range of time-varying exposure profiles can be programmed and then executed by four precision pumps
• real-time measurement of vital signs and exposure parameters (particulate concentration, gas temperature, oxygen or CO2 concentrations…)
• 21 CFR Part 11-compliance with the glp software extension

exposure subsystems

nose-only exposure towers

• standard models accommodate up to 48 mice or 16 rats with a single base unit
• low internal volume minimizes unintentional dilution and dramatically reduces ramp-up and wash-out times
• furthermore, low volume also permits rapid alternation between gas mixtures or concentration levels, e.g. rapid alternation between cigarette smoke and air to mimic the human smoking process

whole-body exposure chambers

• whole-body chambers available in two sizes: 5 L for 12-24 mice; 10 L for 3-6 rats
• up to four chambers per base unit
• chambers designed to achieve a uniform distribution of gases and particulates throughout the entire chamber

masks & third-party setups

• inExpose can be used with face masks for experiments with larger animals or other third- party exposure devices

in vitro cell cultures exposure

• The compact size of most in vitro exposure setups and the high sensitivity of cells to toxins makes inExpose ideally suited to this application.

nebulization

• inExpose is suitable for inhalation exposure studies using a wide range of aerosolized liquids, solutions, suspensions and powders as well as vapours and gases
• up to four nebulizers may be integrated with each inExpose base unit
• nebulization rate and timing can be individually controlled for each nebulizer

cigarette smoke

The standard inExpose system can be easily configured to study acute, sub-chronic and chronic animal models of cigarette-smoking induced lung disease.

Additional key components of an inExpose setup configured for smoking exposure are some or all of the following:

• "smoking" device

• cigarette smoking robot - the smoking device for detailed study of the effects of smoking. The robot has a carousel that holds up to 24 cigarettes. Any combination of sequential or parallel smoking can be programmed. Lighting, end-detection and ejection are fully automatic.
The robot permits typical smoke concentrations to be maintained for up to four hours without user intervention.

• single cigarette chamber - for applications requiring less smoke and shorter exposure times.

• buffer chamber - can be used, if desired, to dilute smoke, for example to extend the duration of a puff.

• bias-flow pumps (1 to 4) - each may be connected to a smoking device or a buffer chamber.

• transducer - for measurement of particle parameters (this is important because cigarettes exhibit variable burning characteristics depending on their age, storage humidity, how they were lit and the ambient conditions).

• software - flexiWare software controls the system and monitors exposure parameters.

The key features of of an inExpose setup configured for smoking exposure are:

• virtually any desired puffing profile can be created, including published standards (predefined templates in software) and custom profiles for special applications
• minimal dead volume to minimize unintentional dilution and ensure effective smoke delivery.
• main stream or side stream smoke exposure
• 8 channels

Depending on your requirements, you may also be interested in measuring lung mechanics of animals exposed to cigarette smoke, e.g. for full phenotyping of animal models of COPD. The flexiVENT system may be used for this purpose.

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